Conclusions Conductive paper sheets were successfully synthesized in thepresence of polyaniline (PANI), polystyrene (PS), and sliver nitrate(AgNO3) with different concentrations during oxidative polymer-ization reaction of aniline monomer. The prepared paper sheets were evaluated by SEM, XRD and IR, the mechanical properties of the prepared paper sheets were evaluated and showed an improvement by added PS and Ag-NPs compare by blank paper sheet Additionally, the electrical conductivity of the paper sheet was enhanced by addition of PANI and Ag-NPs. Moreover, the biological studies (such as cellulases assay, microorganism and culture condition and detection of the released Ag-NPs) were evaluated for the prepared paper sheets and displayed good antibacterial properties against gram positive and gram negative bacteria. Consequently,the prepared paper sheet can be used as packaging materials.