In a sterile condition and near a flame, 1gr of each sample was cut with a sterile scalpel, was mixed with 9 CC Gulity (s, aureuse enrichment, Selective Media/ Merck, Germany) containing 0.1% potassium tellurite and the solution was suspended. Tubes were incubated at 37 ̊c for 24-48 hours. After passing the desired time, tubes that had deposits or were black, were cultured in Baird Parker (Merck,Germany) With 0.1% potassium tellurite and egg emulsion, though Linear Culture method; afterward, they were incubated at 37 ̊c for 24- 48 hours in sterile conditions.