Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester efflu-
ent (ADE) from cattle manure digestion and compared with algal cultivation in Bold’s Basal Medium
(BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280 mg/L after 21 days
in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a signif-
icant species bias to synthetic compared to organic waste-based media. The highest accumulation of pro-
tein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW),
respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest
that biomass from UTEX 1230 in ADE may serve as a candidate alga and growt h system combination sus-
tainable for animal feed production considering high yields of protein, starch and low lipid accumul ation.