IBM examination of the virus preparation concentrated from petioles of grapevine cv. Muscat d' Alexandrie showed that the grapevine was infected with GLRa V-2 and GLRa V-3. N. benthamiana plants inoculated with viruses from this grapevine developed chlorotic local lesions and systemic vein clearing (Fig. 1A) in approximately 15 and 22 days respectively. Within 30 days a general yellowing of the leaves, necrosis of the stem from the base of the plant upwards, wilting and then death of infected plants were observed. In further mechanical transmission of the virus from N. benthamiana plants, local and systemic symptoms appeared in about 8 and 15 days respectively.