The 600-O-acetylglucosides and the 600-O-malonylglucosides, and the β-glucosidesand the aglycones were used as standards of the isoflavones.
For the determination of b-glucosidase activity, p-nitrophenyl-b-D-glucopyranoside
(p-NPG) was used as the substrate, and for the construction of the calibration
curve was used chromogenic reagent p-nitrophenol (p-NP).
The reagents used in the analysis were analytical grade or liquid chromatography grade.