This study investigated the effect of a modified yeast cell wall extract preparation (YCW) on the excretion of aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) in feces, urine, and milk of dairy ewes fed an aflatoxin-contaminated diet. Sixteen ewes in mid-lactation were assigned to 4 treatment groups: control, AF (60 μg of AFB1/kg of feed), YCW (2 g/kg of feed), and AF+YCW.The trial consisted of a short-term (3-d) exposure period followed by a long-term (21-d) exposure period.
At the end of each exposure period, milk, urine, and feces were collected over 72 h. The treatments did not affect feed intake, milk production, milk composition,or body weight. The presence of AFM1 was detected in all matrices, whereas AFB1 was only present in feces.Daily excretion was higher following long-term exposure and reached 26.9 μg of AFB1/d in feces, 37.2 μg
of AFM1/d in feces, and 10.7 μg of AFM1/d in urine.
Supplementation with YCW was effective in increasing
aflatoxin excretion in feces in the long-term exposure
(up to 156% increase). The effect was accompanied by
a trend of decreasing urinary excretion of AFM1. In
contrast, the addition of YCW to the contaminated
diet did not affect the transfer of aflatoxins from feed
to milk under the present experimental conditions with
low-producing ewes. The transfer rates of AFM1 in
milk ranged from 0.24 to 0.54%. In conclusion, feed
supplementation with YCW reduced the absorption
of AFB1 and increased the elimination of AFB1 and
AFM1 in ewe feces. Yeast cell wall extract could be
used to protect ruminants from chronic exposure to
aflatoxins present in feeds.
This study investigated the effect of a modified yeast cell wall extract preparation (YCW) on the excretion of aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) in feces, urine, and milk of dairy ewes fed an aflatoxin-contaminated diet. Sixteen ewes in mid-lactation were assigned to 4 treatment groups: control, AF (60 μg of AFB1/kg of feed), YCW (2 g/kg of feed), and AF+YCW.The trial consisted of a short-term (3-d) exposure period followed by a long-term (21-d) exposure period.At the end of each exposure period, milk, urine, and feces were collected over 72 h. The treatments did not affect feed intake, milk production, milk composition,or body weight. The presence of AFM1 was detected in all matrices, whereas AFB1 was only present in feces.Daily excretion was higher following long-term exposure and reached 26.9 μg of AFB1/d in feces, 37.2 μgof AFM1/d in feces, and 10.7 μg of AFM1/d in urine.Supplementation with YCW was effective in increasingaflatoxin excretion in feces in the long-term exposure(up to 156% increase). The effect was accompanied bya trend of decreasing urinary excretion of AFM1. Incontrast, the addition of YCW to the contaminateddiet did not affect the transfer of aflatoxins from feedto milk under the present experimental conditions withlow-producing ewes. The transfer rates of AFM1 inmilk ranged from 0.24 to 0.54%. In conclusion, feedsupplementation with YCW reduced the absorptionof AFB1 and increased the elimination of AFB1 andAFM1 in ewe feces. Yeast cell wall extract could beused to protect ruminants from chronic exposure toaflatoxins present in feeds.
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