Chromatographic peakswereidentifiedbasedonretention
times, massspectraofauthenticcompoundswhenavailable,
Adams (2007), NIST98andWILEY275librariesofmassspectra
and publisheddata(Adams, 2007).
2.6. Quantificationandsemi-quantificationbygas
chromatography-flame ionizationdetector(GC-FID)
Essential oilcompositionwasquantifiedusingaHewlett-
Packard 5890IIGC,equippedwithflameionizationdetector(FID)
and anon-polarcolumnRtX-5MS(30m×0.25mmi.d.×0.25m
film thickness).Thevolumeofthesamplesmanuallyinjectedwas
1L (insplitlessmode).Heliumwasusedasthecarriergaswith
a flowrateof1mL/min.Theoventemperaturewasprogrammed
in thesamewayasforGC–MSanalysis.Theinjectoranddetector
temperatures were220 ◦C and300 ◦C respectively.Quantification
was carriedoutbasedonasix-pointcalibrationcurveforeach
compound understudy.
The semi-quantificationprocedurewasperformedbycompar-
ing theareasofpeaks,inordertodeterminetheproportionof
each compoundpresentinthearomaticprofilewithrespecttothe
technique usedforisolatingtheessentialoilfromtheplanttissues.
Chromatographic peakswereidentifiedbasedonretentiontimes, massspectraofauthenticcompoundswhenavailable,Adams (2007), NIST98andWILEY275librariesofmassspectraand publisheddata(Adams, 2007).2.6. Quantificationandsemi-quantificationbygaschromatography-flame ionizationdetector(GC-FID)Essential oilcompositionwasquantifiedusingaHewlett-Packard 5890IIGC,equippedwithflameionizationdetector(FID)and anon-polarcolumnRtX-5MS(30m×0.25mmi.d.×0.25mfilm thickness).Thevolumeofthesamplesmanuallyinjectedwas1L (insplitlessmode).Heliumwasusedasthecarriergaswitha flowrateof1mL/min.Theoventemperaturewasprogrammedin thesamewayasforGC–MSanalysis.Theinjectoranddetectortemperatures were220 ◦C and300 ◦C respectively.Quantificationwas carriedoutbasedonasix-pointcalibrationcurveforeachcompound understudy.The semi-quantificationprocedurewasperformedbycompar-ing theareasofpeaks,inordertodeterminetheproportionofeach compoundpresentinthearomaticprofilewithrespecttothetechnique usedforisolatingtheessentialoilfromtheplanttissues.
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