Flies were left in the cups for seven days at which point the fruit was taken out and aged for an additional two days before being assessed for the presence of drosophila larvae and pupae using a boil method for larval detection. To conduct the boil test, fruit were covered withwater in a 473ml plastic container (Gordon Food Service) and heated in a 900 W microwave so that they boiled for 1 min. Fruit were then lightly mashed over a hardware cloth screen (0.64 cm hole size) over a dark colored tray,
and rinsed with 500 ml of cold water poured over the crushed berries.