Therefore, to minimize the bending of the dsDNA and complete the hybridization, we used a 1:10 ratio of the anchoring-to-recognition sequence. As shown in Fig. 2A, after hybridization with rDNA, 5-fold enhancements compared to untreated GaAs were obtained on the GaAs with modification of aDNA (20 mer) and subsequently hybridization with rDNA (20 mer). The enhanced PL emission could be attributed to the structural transformation of flexible single-stranded structure to rigid rod-like duplex, resulting in a different electronic passivation of the GaAs surface.