The killer yeasts W. anomalus (strain 422) and M. guilliermondii
(strain 443) demonstrated in vivo antagonistic activity against C.
gloeosporioides when inoculated 3 h before the phytopathogen. The suspensions of both strains significantly reduced (P
≤
0.05) the
injury caused by C. gloeosporioides in papaya when inoculated 3 h
after the yeast treatments up to 6 d of the experimental period
(Fig. 2).
Inoculation with antagonistic yeast delayed the onset of the
anthracnose symptoms, and the diameter of infection remained
significantly lower (P
≤
0.05) than the control group up to the end
of the experimental period (6 d) and similarly to the fungicide
thiabendazole. At this time, W. anomalus (strain 422) and M. guilliermondii
(strain 443) killer yeast reduced the wound diameter by
24.62% and 20.68%, respectively, when inoculated 3 h prior to the
inoculation with the phytopathogen (Fig. 2).