and ten-fold serially diluted in the 0.1% buffered peptone water. 0.1 ml of each dilution was spread on Palcam agar containing 5 mg/L acriflavin and40 mg/L nalidixic acid and McConkey agar media containing 40 mg/L nalidixic acid that were used to count of L.monocytogenes and E. coli, respectively