Therefore, the aim of this study was to ini ate Annona emarginata in vitro growth. For axillary shoot prolifera on, in vitro nodal segments of 0.5 cm were transferred to MS or WPM supplemented with BA at di erent concentra ons. Nodal segments were also cultured on WPM medium with ranging concentra ons of di erent plant growth regulators, aiming for either shoot elonga on or roo ng.