Firstly, three slices were randomly chosen and foreordain for
the SEM analyses. Furthermore, the strips with 1 mm thickness
were taken from each sample (carrot slice) and put into the vacuum
chamber. The SEM images were taken using 50 magnification.
Image analysis was conducted using MultiScan v 13.11 software
(CSS, Warsaw, Poland, 2008). Measurements of the cross-section
area were carried out for each cell. The histograms of the measured
values were calculated using Microsoft Excel 2010.