Chemical Analysis. Dietary Fiber. The AOAC enzymaticgravimetric method (8) was used, modified by using dialysis
against water instead of ethanol precipitation of soluble dietary
fiber (9) for dietary fiber analysis. After enzymatic hydrolysis
of digestible components, insoluble dietary fiber (iDF) and
soluble dietary fiber (sDF) fractions were separated and
chemically hydrolyzed. The remaining residue was gravimetrically quantified as Klason lignin (KL). Constituent neutral
sugars (NSs) and uronic acids (UAs) were quantified in the
hydrolysates. UAs were quantified spectrophotometrically by
the Scott method (10). NSs were determined by gas chromatography as alditol acetates (11)
Insoluble DF was calculated as (NS+UA+KL), and sDF
was calculated as (NS+UA). Total DF (tDF) was calculated
as (iDF+sDF).
ndigestible Fraction.In vitro determination of the indigestible fraction (IF) followed the procedure established at our
laboratory (12). Briefly, after enzymatic treatments to remove
digestible components, insoluble indigestible fraction (iIF) and
soluble indigestible fraction (sIF) were obtained. The remaining residue was gravimetrically quantified as iIF, and sIF was
measured with dinitrosalicylic acid. Total IF (tIF) was calculated as (iIF +sIF).
Chemical Analysis. Dietary Fiber. The AOAC enzymaticgravimetric method (8) was used, modified by using dialysis
against water instead of ethanol precipitation of soluble dietary
fiber (9) for dietary fiber analysis. After enzymatic hydrolysis
of digestible components, insoluble dietary fiber (iDF) and
soluble dietary fiber (sDF) fractions were separated and
chemically hydrolyzed. The remaining residue was gravimetrically quantified as Klason lignin (KL). Constituent neutral
sugars (NSs) and uronic acids (UAs) were quantified in the
hydrolysates. UAs were quantified spectrophotometrically by
the Scott method (10). NSs were determined by gas chromatography as alditol acetates (11)
Insoluble DF was calculated as (NS+UA+KL), and sDF
was calculated as (NS+UA). Total DF (tDF) was calculated
as (iDF+sDF).
ndigestible Fraction.In vitro determination of the indigestible fraction (IF) followed the procedure established at our
laboratory (12). Briefly, after enzymatic treatments to remove
digestible components, insoluble indigestible fraction (iIF) and
soluble indigestible fraction (sIF) were obtained. The remaining residue was gravimetrically quantified as iIF, and sIF was
measured with dinitrosalicylic acid. Total IF (tIF) was calculated as (iIF +sIF).
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