. Inoculations were done on PDA medium [18] with streptomycin (300 mg/l) to avoid bacterial contamination and incubated at 28 ± 2 °C for 7 days. The isolated cultures were confirmed as L. theobromae by the morphological and microscopical descriptions provided [15]. Pure fungal cultures were isolated and maintained on PDA slants. The cultures of sapstain fungus (L. theobromae) thus obtained were used for further studies.