- DNA Extraction from Young leaves Tissues
- PCR Amplification of RAPD and SSR markers were considered to be suitable for this analysis. Five RAPD markers and five SSR markers were used.
- The amplified DNA products were resolved on 1.2% agarose gel of electrophoresis, visualized by ethidium bromide staining and photographed under UV light.
- Then, DNA bands showed up.
- After that cluster analysis was done by UPGMA. The resulting clusters were represented in the form of phylogenetic tree dendrogram.