fully developed leaves (FDL) or undeveloped leaves (UDL) excised from 6 to 8 week old Val and BTC seedlings were cut into leaf discs of about 1.0 cm2 in size, and cultured in 75-mm Petri Dishes containing 20 mL of MT basal medium (control) or four shoot-inducing media (SIM): SIM1 MT + 0.5 mg L-1 BA + 0.5 mg L1 Kinetin + 0.1 mg L1 NAA (Guo et al., 2002);