The determination is achieved by combining a White Light Reflectance Spectroscopy (WLRS) sensing platform with a biochip bearing discrete areas spotted with the analyte specific antibodies. Measurement is accomplished in real-time by precise movement of the sensing surface with respect to the reflection probe used for the white light illumination of the biochip and collection of the reflected light. The WLRS sensing approach has been previously exploited by our group for the real-time and label-free immunochemical determination of different biomarkers such as the complement factor C3b (Petrou et al., 2007) and the total- and free-form of prostate specific antigen in human serum samples (Koukouvinos et al., 2015).