Total of 60 kg of grapes were collected and 20 kg were use for each different vinification: conventional vinification of red wine (maceration of the pomace lasted for 10 d), prolong maceration (maceration of the pomace lasted for 21 d), and wine with oenological enzyme added(maceration of the pomace lasted for 10 dd and addition of 3 g/100 kg of enological enzyme).