Contact lens cases for amoeba cultivation
The lens storage cases were opened under aseptic conditions. In the storage cases with solution: Samples solutions were collected from 2 sides of the cases and
centrifuged at 2,000 g for 5 mins12. The supernatant were
discarded then 100 μl of the pellet were examined under
light microscope for simple screening. Another drop of 100
μl of the pellet was subjected to culture.