According to the method of Kim et al. (2011), BGRFs were prepared
from L. edodes mushrooms. The mushrooms obtained from
Hanabiotech Ltd. (Gyeonggi-do, Korea) were oven-dried at 55 C for
24 h, ground, and sieved through a 50-mesh screen. The suspensions
of mushroom powder in distilled water (5%, w/v) were agitated for
5 min and filtered with miracloth (Merck KGaA, Darmstadt, Germany).
After the residue was resuspended in distilled water, the
slurrywas autoclaved at 120 C for 10 min. The resultant samplewas
filtered with miracloth and the residue was then freeze-dried.