different morphogenic responses were observed from leaf segments according to type of growth regulator,explant orientation and incubation condition. plant production via direct organogenesis was observed after 30 days culture from the wound surfaces and midvein of explants cultured in the presence of light with the adxial surface in contact with the medium (MSM supplemented with 13.2 and 22 mM BA)(fig.1e),at frequencies of 20 and 33%,respectively,with a mean production of 2 shoots/explant. After 60 day culture,compact,green,non-morphogenic calluses were also observed. However,when cultured with the abaxial surface in contact with the medium leaf segments showed the formation of non-morphogenic compact calluses,with friable regions at the surface,in response to all BA concentration (Fig.1f). Non-morphogenic compact calluses were also observed from cultures maintained in the dark,irrespective of explant orientation.