Principle
Microtitre strip wells are precoated with Dengue virus type-2 antigens, which bind to antibodies in patient's serum.
After washing the wells to remove all unbound antibodies,
horseradish peroxidase
labelled
anti-human IgM conjugate is added.
Dengue virus specific antibody
Wells are washed to remove
unbound
conjugated
The antigen-IgM-conjugate complex is detected by addition of the substrete
Tetramethylbenzidine, which gives a blue reaction product
produce
endpoint