Survival of L. innocua present in a mixed culture, inoculated on the surface of various films and stored at 7 ± 1 C, is shown in Fig. 4. Microbial analysis for Port Salut cheese (with and without film) is presented in Fig. 4a. Again, a cheese without inoculum and without film (CH) and an inoculated cheese without film (ICH), were tested. The inoculated cheese without film showed an initial bacterial count of 4 log CFU/ml which increased its value acquiring at the end of storage a value of 7 log CFU/ml. The CH shows bacterial counts below 10 CFU/ml over the entire storage; this result demonstrated the good quality of the cheese, showing absence of Listeria spp. Covered cheeses with films CNANI, CF and NANI (all inoculated) showed similar bacterial count to that obtained for the cheese CH without inoculum and without film (less than 10 CFU/ml throughout all storage), indicating that these films did not allow the passage of contaminant bacteria. The cheese with the direct application of antimicrobials (DA) reduced the count of L. innocua to a value lower than 10 CFU/ml immediately. Then, the bacteria reassumed its growth reaching a value of 5 log CFU/ml, being 2 log cycles lower than cheese CH.