Experiment 3 Prevention of bacterial contamination in TIB system
The fumigation once a month and daily clean the floor by disinfectant were applied to laboratory. Plant culture media including HF and HF containing PPM (2mL/L) were provided to sugarcane shoots The experiments were carried out for 2 weeks. The observation of bacterial contamination was determined. The decrease of bacterial population was shown in Fig.6 Sugarcanes cultivated in 20 L TIB of HF with and without PPM for 2 weeks were not found any bacterial contamination in both of them.