Complicated solvent systems
The dried material (leaves, roots, barks, wood, resinous secretion of insects etc) are ground to very fine particles.
The crude dried powder is weighed and solvent extracted using Soxhlet Apparatus, Steam Heated Extractor.
Different solvents (such as Acetone, chloroform, ether, n-hexane, alcohol, soda ash, etc.) are used for Extraction.
The process is carried out for 4 hours. The dye extract is evaporated in an evaporating dish over a water bath. After evaporating to dryness, the solute is weighed and the percentage yield is calculated.