5. Add 5 ml of Proteinase K/Cell Lysis Buffer to each labeled 50 ml tube.
6. Trim excess agarose from top of plugs with scalpel, razor blade or similar instrument. Open reusable plug mold and transfer plugs from mold with a 6-mm wide spatula to appropriately labeled tube. If disposable plug molds are used, remove white tape from bottom of mold and push out plug(s) into appropriately labeled tube. Be sure plugs are under buffer and not on side of tube.