The result of the anti-inflammatory test carried out on the crude extract of PIL showed
that the ethanolic extract has significantly reduced the paw volume (edema) only at 300
mg/kg. This indicated possible anti-inflammatory activity of leaves part of the plant at
higher dose. It is ubiquitously known that carrageenan-induced paw oedema involves
many mediators which induce inflammatory reaction in two different phases (26). These
two different phases have caused two peaks which can be clearly observed in the effects
of the control group (Figure 1). The initial phase, which occurs between 0 and 2.5 hours
after the injection of the phlogistic agent, has been attributed to the action of mediators
such as histamine, serotonin and bradykinin on vascular permeability (27)(Maity et al.,
1998). It has been reported that histamine and serotonin are mainly released during first
1.5 hours while bradykinin is released until 2.5 hours after carrageenan injection