Auxin Synthesis
Auxin estimation was carried out by following the
method of Tang and Borner [22]. Briefly, 5 ml tryptone
water was inoculated with bacterial isolates and incubated
at 37ºC for 24 h. After incubation, 2-3 drops of orthophosphoric
acid and 2 ml of Salkowski reagent (4.5 g of FeCl3
per liter in 10.8 M H2SO4) were added to 1 ml supernatant,
and after 30 min of incubation at room temperature, the
color development from yellow to pinkish was monitored at
600 nm by a UV 4000 spectrophotometer.