the samples. Since there were no ions and soluble solutes lost in
the MWB samples (Ramesh et al., 2002; Lin and Brewer, 2005), unlike
in HWB samples, large amounts of un-freezable water and
higher mobility occurred in the frozen MWB samples leading to
the lowest transition temperatures. NOB samples had lower transition
temperature than HWB probably because of loss of soluble
solutes during HWB