Then, the emulsion was heated at 80 C and kept at this temperature until the emulsion breaking point was reached. Afterwards, approximately 1.5 mL of the separated aqueous phase was collected, and zinc concentration was measured by using single line FI-FAAS.
The results obtained are shown in Fig. 3. As it can be seen from the Fig. 3, there is no remarkable variation in the magnitude of the absorbance signals employing different concentrations of Triton X-100 or Triton X-114 in the aqueous solution of zinc used
for EIEB procedure.
This probably happens because the surfactant is not only participating in the extraction process, but also promoting the formation of the water-in-oil emulsions and thus enhances the contact between the acidic aqueous solution and the edible
oils.