Through a funnel containing small amount of anhydrous sodium sulfate on a filter paper
The separating funnel was rinsed with about 2 ml petroleum ether using a pipette.
The Eluent volume was made up to 25 ml with petroleum ether.
Absorbance of the orange-coloured eluent was measured
The flask was capped and gently mixed .
For determination of the carotenoids
The separating funel was rinsed with about 2 ml petroleum ether using a pipette