Fig. 5. Effect of whey and whey proteins on mitochondrial membrane potential and cell viability. Graphs show
mitochondrial membrane potential and cell viability in pGIP/ Neo STC-1 cells (mean ± SEM; n = 6) following 3 h incubations with: (A) and (C): crude yogurt whey (YW) and cheese whey (CW) (5–100 mg/ml), (B) and (D): betalactoglobulin (BLG), alpha-lactalbumin (ALA) and bovine serum albumin (BSA) (0.3125–10 mg/ml). Mitochondrial membrane potential was determined using high content analysis. Cell viability was assessed using Alamar blue. Statistical significance compared with control (one way ANOVA with Tukey’s post hoc test) is indicated by ⁄⁄⁄P < 0.001. Group comparisons (two way ANOVA with Bonferroni post hoc test is indicated as follows: BLG vs ALA: P < 0.05; ALA vs BSA: §§P < 0.01.