Six sterile test tubes with 9 ml sterile nutrient broth were taken. 1ml of different concentration of drug solution was added and 0.1ml inoculum was also added to the test tube aseptically and media blank with the nutrient broth and
the drug solution was also prepared. A positive control, containing media with 0.1ml inoculum was maintained to indicate the growth promotion capacity of the media. Test samples of Staphylococcus epidermidis were incubated at 37°C for 24 hours and those of Propionibacterium acnes were incubated under anaerobic condition in an anaerobic jar (Hi-Media) with gas pack for 48h.