S. thermophilusST1 was grown in 12% RSM at 37 °C for 24 h. The
fermented milk was centrifuged at 8000 ×gfor 10 min at 5 °C. The su-pernatant was adjusted to a pH of 6.5 using 1 M NaOH and thenfiltered
through a 0.22-μmpore-sizefilter (Millipore, Billerica, MA, USA) for
sterilization. The CFS was lyophilized and kept frozen (−80 °C) until
use.