Plant study was carried according to Preethi Ravindran et al (2015) and R.Z Sayyed et al (2005) [12]
[13] with modification. The seeds of Lens Culinaris (Masoor dal i.e. red lentils) and Phaseoluslunatus
(large white Lima Beans) were surface sterilized with Distilled water to remove any surface bacteria.
These seeds were then allowed to germinate on sterile moistened cotton cloth. This was followed by
regular moistening with sterile distilled water. After germination these seeds were collected and
immersed in media containing essential nutrients in the form of salts supplemented with iron. This can
serve as a control. On the other hand some seeds of Lens Culinaris and Phaseoluslunatus were
immersed in same media but under iron limiting conditions. This was followed by incubation on
shaker at RT for 24hrs. Soil was collected and autoclaved to reduce the microbial load. 8-9 germinated
seeds of Lens Culinaris along with 3-4 germinated seeds of Phaseoluslunatus from the flask with iron
limiting conditions were immersed for 10-15 min in siderophore released by the isolate under study.
These treated seeds i.e. form the control medium, iron deprived media and iron deprived along with
Siderophore supplemented conditions were then transferred to the soil and allow to grow for
minimum 7-8 days cycle under pot culture conditions. Growth of the plants was monitored as shoot
length, root length and number of leaves with its colour