Freeze-dried A. bisporus samples with moisture contents corresponding to aw higher than 0.90 were also analyzed. Pre-calculated amount of distilled water was added directly into the freeze-dried powder in weighing bottles and then the bottles were sealed and put in a dry desiccator at 4 °C in a refrigerator (Model SC-230, Haier Group, Qingdao, China) for 24 h before DSC analyzes ( Telis and Sobral, 2001).