Distilled water (5 ml) was added into a control tube. All procedures were conducted at 4 C with the tubes then held in a water bath at 20 C for 30 min. The reaction was then terminated by the addition of methanol. T
Distilled water (5 ml) was added into a controltube. All procedures were conducted at 4 C with the tubes thenheld in a water bath at 20 C for 30 min. The reaction was thenterminated by the addition of methanol. T