following cycling protocol: 5 min at 95C, followed by 35 cycles consisting of 94C for 30 s, 60C for 30 s, 72C for 30 s, and with a final extension of 72C for 10 min.
following cycling protocol: 5 min at 95C, followed by 35 cycles consisting of 94C for 30 s, 60C for 30 s, 72C for 30 s, and with a final extension of 72C for 10 min.