2.4. Biological Activity
The standard disc-agar diffusion method [27] was fol-
lowed to determine the antibacterial and antifungal ac-
tiveity of the synthesized compounds. The tested com-
pounds were dissolved in DMF (which have no inhibit-
tion activity), to get concentrations of 100 g/mL. Uni-
form size filter paper disks (3 disks per compound) were
impregnated by equal volume (0.1 mL) from the specific
concentration of dissolved tested compounds and care-
fully placed on incubated agar surface. After incubation
for 48 h at 37˚C, inhibition of the organisms which evidenced by clear zone surround each disk was measured
and used to calculate mean of inhibition zones.