Tips for Live Cell Imaging:
For samples mounted in aqueous medium (live cells), use the Focus Assist tool to find focus:
Click on the Focus… button.
Follow instructions in the Focus Assist window. Click Start Focus Assist.
Rotate the focus knobs toward you, until the purple bar reaches a maximum position.
Click Stop Focus Assist and close Focus Assist window. Your sample should be in focus.
If you must use eyepieces to focus on your cells, use DIC/Transmitted Light instead of
fluorescence.
To find rare events or dual-labeled cells:
Scan slide with eyepieces using DIC to mark areas that are densely populated.
Switch to software and do a Spiral Mosaic in fluorescence at each marked point to find
rare events or dual-labeled cells.