2.5. Capsaicinoids Analysis byHPLC-DAD. Chromatography conditions were based on the validated method reported
previously [9]. The analyses of capsaicinoids were performed by HPLC-DAD (Agilent 1200, Agilent Technologies Palo
Alto, CA,USA) employing a reversedphase columnKromasil Eternity-5-C18 (4.6 × 150 mm) with Precolumn (SUPELCO
Analytical, Sigma-Aldrich) at 25∘C. Elution was performed with an isocraticmixture of water : acetonitrile 50 : 50. Detection was set at 202, 222, and 280 nm. Injection volume was 20