2.2. Mosquitoes rearing
The larvae of Cx. quinquefasciatus, Ae. aegypti and An. stephensi were maintained in the laboratory at a temperature of (25 ±2) 曟 , (70 ±5)% relative humidity and LD 14:10 h. The different larval instars were maintained in separate enamel containers (25 cm length ×15 cm width ×5 cm depth), at a density of 200 larvae per container. All larvae
were fed 0.4 mL/container of a 5% (w/v) autoclaved suspension
of freeze-dried yeast in distilled water on day 1 and day 2. Larvae were reared in double-distilled sterile water at pH 7.0.To counteract evaporation, water was added daily.