and 0.1 ml of each
dilution was inoculated in appropriate growth media to estimate
the microbial counts: LAB on MRS agar in anaerobic conditions at
30 C for 2 days; Staphylococcus on Manitol Salt Agar (MSA) plates
at 30 C for 2e3 days; Enterobacteriaceae on Violet red bile glucose
agar (VRBG) at 37 C for 24 h; Yeasts on rose bengal chloramphenicol
agar at 25 C for 3e4 days. The results were expressed as
colony-forming units per gram (log cfu g1).