2.6. AllometryThe influence of size variation on the shape variables of males and in females (the allometric effect) was estimated through the determination coefficient by regressing the shape variables onto the estimate of size. For the linear measurement method, the LSR1 and LSR2 were regressed onto the log-size; for the outline method, only the two first PCs of NEF coefficients were regressed onto the semi-major axis of the first ellipse.2.7. SoftwareFor specialized statistical morphometric analyses [29] and contour digitization, we used the free online software XYOM [39]. To perform the ANN-based validated classifica-Insects 2022, 13, x FOR PEER REVIEW 7 of 14tion, we used the multilayer perceptron program written in JavaScript, which is availableat https://www.npmjs.com/package/mlp, accessed on 8 April 2022.