population of longan and postharvest decay Following ozone treatment, 10 replicates from each treatment were sampled immediately after fumigation, and again 3 d after storage at 25 1C and RH ¼ 75%. The sampled fruits were immersed in sterile distilled water and shaken for 30 min. For each treatment, a sample (1 ml) of the suspension was spread over PDA medium. The PDA
plates were incubated at 25 1C for 3 d and the survival of microorganisms was expressed as the mean number of colony forming units (cfu ml_1). After 3 d of incubation, the number of fruits showing decay, and the percentage of
disease incidence was assessed as a lesion area of fungal infection on the fruit surface.