Processing:
Processing of biomass: The Spirogyra filaments were sun dried. The dried Spirogyra biomass was grinded and
filtered through 1mm sieve. Fine powder of Spirogyra biomass obtained was used for saccharification and
fermentation experiments.
Chemical pre-treatment of Spirogyra biomass: 50% Spirogyra biomass was chemically treated with
1%NaOH for a period of 2 hrs (12, 13).