3.3.2. Effect of Trichoderma spp. culture filtrates on the mycelial
growth and spore germination of F. solani
Trichoderma spp. culture filtrates (nonvolatile components) at
12.5%, 25%, and 50% concentrations (v/v) exhibited concentrationdependent inhibition of F. solani mycelial growth. However, the filtrate of T. atroviridae culture showed more potency at the respective
tested concentrations with 42.14%, 72.72%, and 80% mycelial
growth inhibition against 37.66%, 69.09%, and 72.72% for Trichoderma sp. (Fig. 4).
At the same concentrations, BCAs culture filtrates potently
inhibited F. solani conidia germination as shown in Fig. 4. The effect
was concentration-dependent and showed that the culture filtrate
of T. atroviridae could exert 100% inhibition at 25% and 50%
concentrations (v/v), against 11.11% and 33.33% respectively for
Trichoderma sp. at the same concentrations.
3.3.3. Effect of ethyl acetate extract of T. atroviridae culture filtrate on
F. solani conidia germination
The ethyl acetate extract of T. atroviridae culture filtrate exerted
concentration-dependent inhibitory effect on F. solani conidia germination (Fig. 5). The minimum extract concentration that
achieved 100% inhibition (MIC) of F. solani conidia germination
was found to be 0.66 mg/mL.