Soybean (Glycine max (L.) Merr.), sesame (Sesamum indicum L.) and Job‟s Tears (Coix lacryma-jobi L.) are widely used in food/beverage industry because they are high content of dietary fibers, proteins, minerals and vitamins. Additionally, the cereals can be applied as source of non-digestible oligosaccharides that can function as prebiotic, stimulating the growth of beneficial bacteria in the colon (Mussatto and Mancilha, 2007). Therefore, it would be desirable to investigate how the cereals affected the viability of L. plantarum under gastrointestinal tract conditions. In our laboratory, thirteen strains of L. plantarum were isolated from fermented vegetable and exhibited high antimicrobial activity against human pathogens including Escherichia coli O157:H7 DMST 12743 and Salmonella Typhimurium ATCC 13311. In order to be used as potential probiotics, the strains need to be screened for their capacity of transit tolerance to gastrointestinal tract conditions. Therefore, in the present study, all test strains were evaluated for their viability in simulated gastric condition (pH 2.0) and small intestinal tract condition (pH 8.0 with 0.45% bile salt). Moreover, the effect of soybean, sesame and Job‟s Tears on simulated gastrointestinal tract tolerance of all test strains was also investigated.