Well developed in vitro mico shoots after attaining the
size of 5.0 cm were shifted for rooting. For in vitro rooting,
MS medium was supplemented with NAA ranging from
1.0 to 10 mg/L. It was observed that the best response
(80%) for rooting was obtained when 10 mg/L of NAA
was used (Figure 1 h). By decreasing the concentration
of NAA, root induction response was decreased and in
1.0 mg/L of NAA, very poor results were obtained (Table
3). Best rooting response was obtained on MS medium
containing 10 mg/L NAA. For acclimatization, different
media were used that is, autoclaved sand, compost and
coco peat. The best response of hardening was noticed
in autoclaved sand.